Molecular Mechanism of Tinospora crispa on Herb-Drug Interaction in Rat Hepatocytes

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Tin, T.P
Lam, C.K
Hussin A. Hj

Abstract

Tinospora crispa (Family: Menispermaceae) has been used locally as a folk medicine for diabetes mellitus. The objective of this study is to elucidate the effects of T. crispa on the molecular mechanism of aminopyrine metabolism in rat hepatocytes. A total of two inhibitors of the second messenger system, namely IBMX and KT-5720 were investigated on the possible pathway that could mediate the effects of T. crispa on hepatic Phase I metabolizing enzymes. Normal old male Sprague Dawley rats (n = 6) were used in this study. Isolated hepatocytes cells were prepared using liver perfusion technique [1]. The effect of T. crispa on aminopyrine N-demethylase activity was determined in the absence or presence of inhibitors by measuring the quantity of formaldehyde formed using the method of Nash [2]. The findings showed that T. crispa may act via the cAMP pathway at lower concentrations, ranging from 1.0, 10, and 100 ng/ml, but gave paradoxical results at higher concentrations (0.001 - 1.0 mg/ml).

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How to Cite
T.P, T., C.K, L., & A. Hj, H. (2005). Molecular Mechanism of Tinospora crispa on Herb-Drug Interaction in Rat Hepatocytes. Malaysian Journal of Science, 24(1), 229–232. Retrieved from http://jice.um.edu.my/index.php/MJS/article/view/8983
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Original Articles