Investigation on The Stem Bark of Trema orientalis

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Abd Malek S.N
Lai, H.S
Muhammad N
Mohamad Ali N.A
Perry, N


A chemical investigation was conducted on the ethyl acetate-soluble fraction of the stem bark of Trema orientalis. This led to the isolation of seven methyl ester of fatty acids hexadecanoic, (Z,Z)-9,12-octadecadienoic, (Z)-9-octadecenoic, octadecanoic, eicosanoic, docosanoic and tetracosanoic. Two triterpenoids identified as β-sitosterol and 3β-acetoxyurs-12-en-28-oic acid were isolated. The methyl ester of fatty acids were identified by GC-MS while the triterpenoid compounds were identified using NMR and infrared spectroscopy and mass spectrometry. The identification of 3β-acetoxyurs-12-en-28-oic acid was verified by comparing it to the authentic 3β-acetoxyurs-12-en-28-oic acid synthesized from the acetylation of ursolic acid.


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Malek S.N, A., H.S, L., N, M., Ali N.A, M., & N, P. (2005). Investigation on The Stem Bark of Trema orientalis. Malaysian Journal of Science, 24(1), 113–119. Retrieved from
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